Food is an important commodity and is required for survival and healthy living in humans. However, poor hygiene
and unsanitary practices by food handlers tend to expose the foods to microbial contamination thereby predisposing
consumers to different diseases. This study aimed at determining the antibiogram and multidrug resistance profile of
bacteria isolated from food samples purchased from two different cafeterias within a Tertiary Institution in Ibadan,
Nigeria. Microorganisms were isolated from food samples using appropriate agar media following standard microbiological procedures. Pure cultures were obtained and identified using different morphological and biochemical tests and thereafter subjected to different antibiotics such as Ciprofloxacin, Sulfamethoxazole/Trimethoprim, Amoxicillin, Tigecycline and Imipinem. The total heterotrophic, coliform and Salmonella-Shigella counts ranged between 0.46 × 104 CFU/mL, 2.56 × 104 CFU/mL and 6.10 × 104 CFU/mL respectively, for both cafeterias. A total of 14 bacterial species were isolated and they belonged to the species E. coli, Staphylococcus aureus, Salmonella spp., Pseudomonas aeruginosa, Proteus sp. and Klebsiella sp. The antibiotics susceptibility revealed that 7.1%, 21.4% and 71.4% of the isolates showed resistance to Imipenem, Ciprofloxacin and Trimethoprim/Sulfamethoxazole respectively, while all of the isolates were resistant to Tigecycline and Amoxicillin. However, 78.6% of the isolates
were multidrug-resistant. The result of this study has shown that the food samples were contaminated with pathogenic microorganisms which also exhibited antibiotic resistance to some antibiotics used in medicine and this could pose a serious health risk to humans when consumed.